SIRT2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54053
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54053-0.1ML0.1 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 1D4-H11-H11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the SIRT2 antibody at -20oC.

Images

1 / 3
ICC/IF analysis of HeLa cells, fixed fixed by anhydrous methanol at -20oC, using SIRT2 antibody at 1:50 (green) and Histone H3.1 (pSer10) antibody at 1:200 (red).
2 / 3
Western blot testing of 1) rat PC12, 2) rat brain and 3) mouse brain lysates using SIRT2 antibody at 1:1000. Predicted molecular weight: multiple isoforms between 36-43 kDa.
3 / 3
Western blot testing of 1) rat cerebral cortex and 2) rat brain lysates using SIRT2 antibody at 1:1000. Predicted molecular weight: multiple isoforms between 36-43 kDa.
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ICC/IF analysis of HeLa cells, fixed fixed by anhydrous methanol at -20oC, using SIRT2 antibody at 1:50 (green) and Histone H3.1 (pSer10) antibody at 1:200 (red).
Western blot testing of 1) rat PC12, 2) rat brain and 3) mouse brain lysates using SIRT2 antibody at 1:1000. Predicted molecular weight: multiple isoforms between 36-43 kDa.
Western blot testing of 1) rat cerebral cortex and 2) rat brain lysates using SIRT2 antibody at 1:1000. Predicted molecular weight: multiple isoforms between 36-43 kDa.

Documents

Further Information

Application Details:
Western blot: 1:1000,ICC/Immunofluorescence: 1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the SIRT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sirtuin 2 is an NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors. Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. Plays a major role in the control of cell cycle progression and genomic stability. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant protein was used as the immunogen for this SIRT2 antibody.
Limitation:
This SIRT2 antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
Q8IXJ6