CD1b Antibody

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Functional studies (order BSA/sodium azide-free format)

Optimal dilution of the CD1b antibody should be determined by the researcher.

The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin (Workshop IV; Code T015). It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Stimulated human leukocytes were used as the immunogen for the CD1b antibody.

This CD1b antibody is available for research use only.

Cell surface, cytoplasmic

PEG precipitation

P29016