CD81 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3485SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3485SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1.3.3.22
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD81 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.~
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IHC testing of FFPE human lymph node with CD81 a
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IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.~
IHC testing of FFPE human lymph node with CD81 a

Documents

Further Information

Application Details:
Functional studies (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml for 2 hours at RT,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD81 antibody should be determined by the researcher.
Description:
This mAb recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full length human protein was used as the immunogen for the CD81 antibody.
Limitation:
This CD81 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Uniprot #:
P60033