MVP Antibody / Major Vault Protein
| Code | Size | Price |
|---|
| NSJ-V3042SAF-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1014
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store the Major Vault Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the Major Vault Protein antibody should be determined by the researcher.
Description:
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the Major Vault Protein antibody.
Limitation:
This Major Vault Protein antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q14764