Resorufin-beta-D-galactopyranoside
| Code | Size | Price |
|---|
| CDX-R0005-M010 | 10 mg | £92.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
Short term: +4°C, Long term: -20°C
Documents
Further Information
Alternate Names/Synonyms:
3-Phenoxazone 7-(beta-D-galactopyranoside)
Appearance:
Orange to brown solid.
CAS:
95079-19-9
EClass:
32160000
Form (Short):
solid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C18H17NO8/c20-7-14-15(22)16(23)17(24)18(27-14)25-9-2-4-11-13(6-9)26-12-5-8(21)1-3-10(12)19-11/h1-6,14-18,20,22-24H,7H2/t14-,15+,16+,17-,18-/m1/s1
InChiKey:
QULZFZMEBOATFS-DISONHOPSA-N
Long Description:
Chemical. CAS: 95079-19-9. Formula: C18H17NO8. MW: BD9837. Resorufin beta-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. Upon enzymatic cleavage by the enzymes, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=584nm. It is used in several applications, specifically in compound screens, where it is advantageous because it is less prone to compound autofluorescence, and thus produces less false positives. It is widely used as substrate in high throughput screening of galactosidase inhibitors.
MDL:
MFCD00064055
Molecular Formula:
C18H17NO8
Molecular Weight:
375.33
Package Type:
Vial
Product Description:
Resorufin beta-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. Upon enzymatic cleavage by the enzymes, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=584nm. It is used in several applications, specifically in compound screens, where it is advantageous because it is less prone to compound autofluorescence, and thus produces less false positives. It is widely used as substrate in high throughput screening of galactosidase inhibitors.
Purity:
>97% (TLC)
SMILES:
O=C1C=C2OC3=CC(O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)=CC=C3N=C2C=C1
Solubility Chemicals:
Soluble in DMSO (20mg/ml).
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) J. Hofmann & M. Sernetz; Anal. Chim. Acta 163, 67 (1984) | (2) K.D. Wittrup & J.E. Bailey; Cytometry 9, 394 (1988) | (3) A.G. Hadd, et al.; Anal. Chem. 69, 3407 (1997) | (4) M.J. Eggertson & D.B. Craig; Biomed. Chromatogr. 13, 516 (1999) | (5) G.K. Shoemaker, et al.; Biochemistry 42, 1707 (2003) | (6) S. Jambovane, et al.; Anal. Chem. 81, 3239 (2009)