ELISA Kit for Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP)
| Code | Size | Price |
|---|
| AEA950Hu-24T | 24T | £270.00 |
Quantity:
| AEA950Hu-48T | 48T | £449.00 |
Quantity:
| AEA950Hu-96T | 96T | £629.00 |
Quantity:
| AEA950Hu-96T*5 | 96T*5 | £2,724.00 |
Quantity:
| AEA950Hu-96T*10 | 96T*10 | £5,118.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Documents
Further Information
Alternative Names:
ADAMTS13; TTP; vWF-cp; vWF-cleaving protease; A Disintegrin And Metalloproteinase With A Thrombospondin Type 1 Motif Member 13
Assay Length:
2h, 30min
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
Detection Range:
3.12-200ng/mL
Item Name:
Von Willebrand Factor Cleaving Protease
Method:
Competitive Inhibition
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
The minimum detectable dose of this kit is typically less than 1.17ng/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP).
No significant cross-reactivity or interference between Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) and analogues was observed.
No significant cross-reactivity or interference between Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Anti-Von Willebrand Factor Cleaving Protease Antibody (Anti-vWFCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Uniprot ID:
Q76LX8