Instant ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)
| Code | Size | Price |
|---|
| IEA133Mu-24T | 24T | £178.00 |
Quantity:
| IEA133Mu-48T | 48T | £289.00 |
Quantity:
| IEA133Mu-96T | 96T | £399.00 |
Quantity:
| IEA133Mu-96T*5 | 96T*5 | £1,688.00 |
Quantity:
| IEA133Mu-96T*10 | 96T*10 | £3,162.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Documents
Further Information
Alternative Names:
DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Assay Length:
1h, 10min
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
Detection Range:
15.6-1,000pg/mL
Item Name:
Tumor Necrosis Factor Alpha
Method:
Double-antibody Sandwich
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Sample Type:
serum, plasma , tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
The minimum detectable dose of this kit is typically less than 5.8pg/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Instant Tumor Necrosis Factor Alpha (TNFa).
No significant cross-reactivity or interference between Instant Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
No significant cross-reactivity or interference between Instant Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Instant Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Instant Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Uniprot ID:
P06804