Human Anti-GAD65 (Anti-Glutamate Decarboxylase 65 Autoantibodies) ELISA Kit
| Code | Size | Price |
|---|
| ELK0506-48T | 48T | £298.00 |
Quantity:
| ELK0506-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
anti-GAD65; anti-Pancreatic Islets And Brain,65kDa; anti-65 kDa glutamic acid decarboxylase; anti-Glutamate decarboxylase 65 kDa isoform
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
3.91-250 IU/mL
Sample Type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
1.31 IU/mL
Standard:
250 IU/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GAD65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GAD65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human Anti-GAD65 in the samples is then determined by comparing the OD of the samples to the standard curve.