Chicken 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
| Code | Size | Price |
|---|
| ELK0592-48T | 48T | £261.00 |
Quantity:
| ELK0592-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Chicken
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Length:
2.5h
Assay Type:
Competitive Inhibition
Detection Range:
93.75-6000 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
26.29 pg/mL
Standard:
6000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Chicken 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.