Cattle GnRH (Gonadotropin Releasing Hormone) ELISA Kit
| Code | Size | Price |
|---|
| ELK0603-48T | 48T | £298.00 |
Quantity:
| ELK0603-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Bovine (Cattle)
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Assay Length:
2.5h
Assay Type:
Competitive Inhibition
Detection Range:
62.50-4000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
23.1 pg/mL
Standard:
4000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cattle GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.