Mouse Anti-IAA (Anti-Insulin Autoantibody) ELISA Kit
| Code | Size | Price |
|---|
| ELK0650-48T | 48T | £298.00 |
Quantity:
| ELK0650-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Anti-Insulin; Antiinsulin; Insulin Antibodies
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.78-50 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
0.31 ng/mL
Standard:
50 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.