Human Anti-GM1 (Anti-Ganglioside Antibody) ELISA Kit
| Code | Size | Price |
|---|
| ELK0676-48T | 48T | £298.00 |
Quantity:
| ELK0676-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
anti-ganglioside (GM1) antibody (IgG)
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
1.25-80 ng/mL
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.57 ng/mL
Standard:
80 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human Anti-GM1 in the samples is then determined by comparing the OD of the samples to the standard curve.