Human IA-2 Ab (Insulinoma associated antigen 2 Autoantibody) ELISA Kit
| Code | Size | Price |
|---|
| ELK0858-48T | 48T | £298.00 |
Quantity:
| ELK0858-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
IA2 Ab
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
62.5-4000 U/mL
Sample Type:
tissue homogenates, cell lysates and other biological fluids
Sensitivity:
27.1 U/mL
Standard:
4000 U/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IA-2 Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IA-2 Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IA-2 Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human IA-2 Ab in the samples is then determined by comparing the OD of the samples to the standard curve.