Rat 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit
| Code | Size | Price |
|---|
| ELK0875-48T | 48T | £261.00 |
Quantity:
| ELK0875-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
25 Hydroxy Vitamin D;
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
3.13-200 ng/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
0.91 ng/mL
Standard:
200 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.