Hamster TNF-a (Tumor Necrosis Factor-alpha) ELISA Kit
| Code | Size | Price |
|---|
| ELK0928-48T | 48T | £261.00 |
Quantity:
| ELK0928-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Hamster
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
3.13-200 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
1.12 pg/mL
Standard:
200 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.