Sheep ADIPOQ (Adiponectin) ELISA Kit
| Code | Size | Price |
|---|
| ELK0997-48T | 48T | £359.00 |
Quantity:
| ELK0997-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Sheep
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
ADP; GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
3.13-200 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
1.15 ng/mL
Standard:
200 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep ADIPOQ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep ADIPOQ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep ADIPOQ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Sheep ADIPOQ in the samples is then determined by comparing the OD of the samples to the standard curve.