Recombinant S. hygroscopicus BAR
| Code | Size | Price |
|---|
| ELK-EPT071-50ug | 50ug | £557.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Bacteria
Regulatory Status: RUO
Shipping:
The product is shipped at ambient temperature.
Storage:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Accession:
P16426
Alternative Names:
Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar
Apparent Molecular Weight:
18-20 KDa, reducing conditions
Background:
Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
Endotoxin:
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Molecular Weight:
20.6 KDa
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Redissolve:
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100ug/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.?