Recombinant 2019-nCoV Papain-Like Protease
| Code | Size | Price |
|---|
| ELK-EPT114-50ug | 50ug | £774.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Virus
Regulatory Status: RUO
Shipping:
The product is shipped on dry ice/polar packs.
Storage:
Store at -70°C, stable for 6 months after receipt. Store at -70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Further Information
Accession:
QHD43415.1
Alternative Names:
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
Apparent Molecular Weight:
34 KDa, reducing conditions
Background:
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
Biological Activity:
Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol/min/ug.
Molecular Weight:
35.8 KDa
Purity:
Greater than 95% as determined by reducing SDS-PAGE.