SPHK1 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6578
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6578-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SPHK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat liver, 2) rat kidney, 3) rat RH35, 4) mouse liver, 5) mouse kidney and 6) mouse Neuro-2a cell lysate with SPHK1 antibody. Expected molecular weight: 43-51 kDa (multiple isoforms).
2 / 15
Western blot testing of human 1) HepG2, 2) MCF7, 3) A431, 4) PC-3, 5) HeLa, 6) U-2 OS and 7) Caco-2 cell lysate with SPHK1 antibody. Expected molecular weight: 43-51 kDa (multiple isoforms).
3 / 15
Immunofluorescent staining of FFPE human HepG2 cells with SPHK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 15
IHC staining of FFPE human placental tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 15
IHC staining of FFPE human lung cancer tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 15
IHC staining of FFPE human liver cancer tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 15
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 15
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 15
IHC staining of FFPE human papillary thyroid carcinoma tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 15
IHC staining of FFPE human Hashintoto's thyroditis tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 15
IHC staining of FFPE human gastric cancer tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 15
IHC staining of FFPE mouse brain tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
13 / 15
IHC staining of FFPE rat brain tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
14 / 15
IHC staining of FFPE rat lung tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
15 / 15
Flow cytometry testing of human A431 cells with SPHK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SPHK1 antibody.
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Western blot testing of 1) rat liver, 2) rat kidney, 3) rat RH35, 4) mouse liver, 5) mouse kidney and 6) mouse Neuro-2a cell lysate with SPHK1 antibody. Expected molecular weight: 43-51 kDa (multiple isoforms).
Western blot testing of human 1) HepG2, 2) MCF7, 3) A431, 4) PC-3, 5) HeLa, 6) U-2 OS and 7) Caco-2 cell lysate with SPHK1 antibody. Expected molecular weight: 43-51 kDa (multiple isoforms).
Immunofluorescent staining of FFPE human HepG2 cells with SPHK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human papillary thyroid carcinoma tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human Hashintoto's thyroditis tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung tissue with SPHK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with SPHK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SPHK1 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SPHK1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R16-H267) was used as the immunogen for the SPHK1 antibody.
Limitation:
This SPHK1 antibody is available for research use only.
Localization:
Nuclear, cytoplasm, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
Q9NYA1