TAF8 Antibody / TBN (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6580
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6580-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the TAF8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human Caco-2 cells with TAF8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human HeLa, 2) human ThP-1, 3) rat heart, 4) rat testis, 5) rat PC-12, 6) mouse heart, 7) mouse testis, 8) mouse lung and 9) mouse RAW264.7 cell lysate with TAF8 antibody. Expected molecular weight: 34-43 kDa.
3 / 4
Flow cytometry testing of human HL60 cells with TAF8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAF8 antibody.
4 / 4
Flow cytometry testing of human A431 cells with TAF8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAF8 antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with TAF8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human ThP-1, 3) rat heart, 4) rat testis, 5) rat PC-12, 6) mouse heart, 7) mouse testis, 8) mouse lung and 9) mouse RAW264.7 cell lysate with TAF8 antibody. Expected molecular weight: 34-43 kDa.
Flow cytometry testing of human HL60 cells with TAF8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAF8 antibody.
Flow cytometry testing of human A431 cells with TAF8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAF8 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TAF8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Transcription initiation factor TFIID subunit 8 is a protein that in humans is encoded by the TAF8 gene. This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K20-S310) was used as the immunogen for the TAF8 antibody.
Limitation:
This TAF8 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q7Z7C8