Cyclophilin E Antibody / PPIE (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6585
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6585-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 7F2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PPIE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 8
Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) mouse heart, 5) mouse brain, 6) mouse lung, 7) rat RH35 and 8) mouse HEPA1-6 cell lysate with PPIE antibody. Predicted molecular weight ~33 kDa.
2 / 8
Western blot testing of human 1) HEK293, 2) K562, 3) PC-3 and 4) Caco-2 cell lysate with PPIE antibody. Predicted molecular weight ~33 kDa.
3 / 8
IHC staining of FFPE rat heart tissue with PPIE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human placental tissue with PPIE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human colonic adenocarcinoma tissue with PPIE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Immunofluorescent staining of FFPE human HeLa cells with PPIE antibody (Dylight 594-conjugated secondary, red) and Phalloidin-iFluor 488 conjugate (green). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 8
Flow cytometry testing of human JK-1 cells with PPIE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPIE antibody.
8 / 8
Flow cytometry testing of human U937 cells with PPIE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPIE antibody.
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Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) mouse heart, 5) mouse brain, 6) mouse lung, 7) rat RH35 and 8) mouse HEPA1-6 cell lysate with PPIE antibody. Predicted molecular weight ~33 kDa.
Western blot testing of human 1) HEK293, 2) K562, 3) PC-3 and 4) Caco-2 cell lysate with PPIE antibody. Predicted molecular weight ~33 kDa.
IHC staining of FFPE rat heart tissue with PPIE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with PPIE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colonic adenocarcinoma tissue with PPIE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with PPIE antibody (Dylight 594-conjugated secondary, red) and Phalloidin-iFluor 488 conjugate (green). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human JK-1 cells with PPIE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPIE antibody.
Flow cytometry testing of human U937 cells with PPIE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPIE antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PPIE antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-V301) was used as the immunogen for the PPIE antibody.
Limitation:
This PPIE antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9UNP9