FGG Antibody / Fibrinogen gamma chain / FIBG (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6593
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6593-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 5H9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the FGG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human Hep3B cells with FGG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human HepG2 cell lysate with FGG antibody. Predicted molecular weight ~52 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with FGG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGG antibody.
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Immunofluorescent staining of FFPE human Hep3B cells with FGG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human HepG2 cell lysate with FGG antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of human HepG2 cells with FGG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FGG antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the FGG antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Fibrinogen gamma chain, also known as FGG, is a human gene found on Chromosome 4. The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
N-terminal region amino acids IRYLQEIYNSNNQKIVNLKEKVAQLEAQCQE from the human protein were used as the immunogen for the FGG antibody.
Limitation:
This FGG antibody is available for research use only.
Localization:
Secreted
Purity:
Antigen affinity purified
Uniprot #:
P02679