AASS Antibody / LKR / SDH (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6595
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6595-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AASS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human Caco-2 cells with AASS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human liver cancer tissue with AASS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human kidney cancer tissue with AASS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human HepG2, 2) human HEK293, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with AASS antibody. Predicted molecular weight ~102 kDa.
5 / 5
Flow cytometry testing of human Jurkat cells with AASS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AASS antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with AASS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with AASS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney cancer tissue with AASS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HepG2, 2) human HEK293, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with AASS antibody. Predicted molecular weight ~102 kDa.
Flow cytometry testing of human Jurkat cells with AASS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AASS antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AASS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Alpha-aminoadipic semialdehyde synthase (AASS), also called Lysine ketoglutarate reductase (LKR) and Saccharopine dehydrogenase (SDH) is an enzyme encoded by the AASS gene in humans and is involved in their major lysine degradation pathway. This gene encodes a bifunctional enzyme that catalyzes the first two steps in the mammalian lysine degradation pathway. The N-terminal and the C-terminal portions of this enzyme contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively, resulting in the conversion of lysine to alpha-aminoadipic semialdehyde. Mutations in this gene are associated with familial hyperlysinemia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E37-N865) was used as the immunogen for the AASS antibody.
Limitation:
This AASS antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9UDR5