ADORA2B Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6597
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6597-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Adenosine Receptor A2b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with Adenosine Receptor A2b antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) HEK293 and 2) U-87 MG cell lysate with Adenosine Receptor A2b antibody. Expected molecular weight: 36-40 kDa.
3 / 3
Flow cytometry testing of human U-251 MG cells with Adenosine Receptor A2b antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adenosine Receptor A2b antibody.
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Immunofluorescent staining of FFPE human A431 cells with Adenosine Receptor A2b antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293 and 2) U-87 MG cell lysate with Adenosine Receptor A2b antibody. Expected molecular weight: 36-40 kDa.
Flow cytometry testing of human U-251 MG cells with Adenosine Receptor A2b antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adenosine Receptor A2b antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Adenosine Receptor A2b antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The adenosine A2B receptor, also known as ADORA2B, is a G-protein coupled adenosine receptor, and also denotes the human adenosine A2b receptor gene which encodes it. This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N145-L328) was used as the immunogen for the Adenosine Receptor A2b antibody.
Limitation:
This Adenosine Receptor A2b antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
P29275