NOX2 Antibody / gp91phox (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6611
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6611-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the NOX2/gp91phox antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U937 cells with NOX2/gp91phox antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX2/gp91phox antibody.~
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Western blot testing of human 1) MCF7 and 2) Raji cell lys
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Flow cytometry testing of human U937 cells with NOX2/gp91phox antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX2/gp91phox antibody.~
Western blot testing of human 1) MCF7 and 2) Raji cell lys

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NOX2/gp91phox antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids F416-H527) was used as the immunogen for the NOX2/gp91phox antibody.
Limitation:
This NOX2/gp91phox antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P04839