E2F1 Antibody (Antigen affinity purified)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6615
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6615-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the E2F1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human PC-3 cells with E2F1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= E2F1 antibody.~
2 / 2
Western blot testing of 1) human HeLa, 2) human A431, 3) human Caco-2, 4) ra
❮❯

Flow cytometry testing of human PC-3 cells with E2F1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= E2F1 antibody.~
Western blot testing of 1) human HeLa, 2) human A431, 3) human Caco-2, 4) ra

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of th1e E2F1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product(pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein(pRB).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K89-A328) was used as the immunogen for the E2F1 antibody.
Limitation:
This E2F1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q01094