FAAH Antibody / Fatty-acid amide hydrolase 1 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6625
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6625-100ug100ug£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Fatty-acid amide hydrolase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with Fatty-acid amide hydrolase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with Fatty-acid amide hydrolase 1 antibody. Predicted molecular weight ~63 kDa.
3 / 3
Flow cytometry testing of human ThP-1 cells with Fatty-acid amide hydrolase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fatty-acid amide hydrolase 1 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Fatty-acid amide hydrolase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with Fatty-acid amide hydrolase 1 antibody. Predicted molecular weight ~63 kDa.
Flow cytometry testing of human ThP-1 cells with Fatty-acid amide hydrolase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fatty-acid amide hydrolase 1 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Fatty-acid amide hydrolase 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Fatty acid amide hydrolase or FAAH is a member of the serine hydrolase family of enzymes. This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R34-S579) was used as the immunogen for the Fatty-acid amide hydrolase 1 antibody.
Limitation:
This Fatty-acid amide hydrolase 1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O00519