FKBP10 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6629
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6629-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the FKBP10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with FKBP10 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) A375, 2) 293T and 3) HeLa cell lysate with FKBP10 antibody. Expected molecular weight: 64-78 kDa.
3 / 3
Flow cytometry testing of human A549 cells with FKBP10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FKBP10 antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with FKBP10 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A375, 2) 293T and 3) HeLa cell lysate with FKBP10 antibody. Expected molecular weight: 64-78 kDa.
Flow cytometry testing of human A549 cells with FKBP10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FKBP10 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the FKBP10 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
FK506-binding protein 10 is a protein that in humans is encoded by the FKBP10 gene. The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R29-E532) was used as the immunogen for the FKBP10 antibody.
Limitation:
This FKBP10 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q96AY3