LARP4B Antibody / La-related protein 4B (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6651
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6651-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LARP4B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human liver cancer tissue with LARP4B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human appendiceal adenocarcinoma tissue with LARP4B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human 1) HEK293, 2) Jurkat and 3) K562 cell lysate with LARP4B antibody. Predicted molecular weight ~81 kDa.
4 / 5
Flow cytometry testing of human ThP-1 cells with LARP4B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LARP4B antibody.
5 / 5
Immunofluorescent staining of FFPE human A431 cells with LARP4B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human liver cancer tissue with LARP4B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with LARP4B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEK293, 2) Jurkat and 3) K562 cell lysate with LARP4B antibody. Predicted molecular weight ~81 kDa.
Flow cytometry testing of human ThP-1 cells with LARP4B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LARP4B antibody.
Immunofluorescent staining of FFPE human A431 cells with LARP4B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the LARP4B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes a member of an evolutionarily conserved protein family implicated in RNA metabolism and translation. Members of this family are characterized by the presence of an La motif, which is often located adjacent to one or more RNA recognition motifs (RRM). Together, the two motifs constitute the functional region of the protein and enable its interaction with the RNA substrate. This protein family is divided into five sub-families: the genuine La proteins and four La-related protein (LARP) sub-families. The protein encoded by this gene belongs to LARP sub-family 4. It is a cytoplasmic protein that may play a stimulatory role in translation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R241-K684) was used as the immunogen for the LARP4B antibody.
Limitation:
This LARP4B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q92615