LSR Antibody / Lipolysis-stimulated lipoprotein receptor (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6652
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6652-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LSR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE human liver cancer tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE mouse colon tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat colon tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Immunofluorescent staining of FFPE human A431 cells with LSR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
Western blot testing of 1) human HepG2, 2) human Caco-2, 3) human RT4, 4) rat liver, 5) rat RH35, 6) mouse liver and 7) mouse HEPA1-6 cell lysate with LSR antibody. Predicted molecular weight: 54-71 kDa (multiple isoforms).
7 / 7
Flow cytometry testing of human MCF7 cells with LSR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSR antibody.
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IHC staining of FFPE human liver cancer tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with LSR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with LSR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human Caco-2, 3) human RT4, 4) rat liver, 5) rat RH35, 6) mouse liver and 7) mouse HEPA1-6 cell lysate with LSR antibody. Predicted molecular weight: 54-71 kDa (multiple isoforms).
Flow cytometry testing of human MCF7 cells with LSR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSR antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LSR antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Lipolysis-stimulated lipoprotein receptor is a protein that in humans is encoded by the LSR gene. LSR has a probable role in the clearance of triglyceride-rich lipoprotein from blood. It binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-V649) was used as the immunogen for the LSR antibody.
Limitation:
This LSR antibody is available for research use only.
Localization:
Cell membrane, cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q86X29