NUP210 Antibody / Nuclear pore membrane glycoprotein 210 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6666
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6666-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NUP210 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human liver tissue with NUP210 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human liver tissue with NUP210 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human appendiceal adenocarcinoma tissue with NUP210 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human MCF7 cells with NUP210 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of 1) human MCF7, 2) human HEK293 and 3) rat thymus tissue lysate with NUP210 antibody. Predicted molecular weight ~205 kDa but may be observed at higher molecular weights due to glycosylation.
6 / 6
Flow cytometry testing of human JK-1 cells with NUP210 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUP210 antibody.
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IHC staining of FFPE human liver tissue with NUP210 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver tissue with NUP210 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with NUP210 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with NUP210 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human MCF7, 2) human HEK293 and 3) rat thymus tissue lysate with NUP210 antibody. Predicted molecular weight ~205 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human JK-1 cells with NUP210 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUP210 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NUP210 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R221-K1724) was used as the immunogen for the NUP210 antibody.
Limitation:
This NUP210 antibody is available for research use only.
Localization:
Cytoplasm, nuclear membrane
Purity:
Antigen affinity purified
Uniprot #:
Q8TEM1