PPP2R1A Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6671
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6671-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PPP2R1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A549 cells with PPP2R1A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human 293T cells with PPP2R1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R1A antibody.
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Flow cytometry testing of mouse RAW264.7 cells with PPP2R1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R1A antibody.
4 / 8
Flow cytometry testing of rat C6 cells with PPP2R1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R1A antibody.
5 / 8
Western blot testing of 1) human HepG2, 2) rat kidney, 3) mouse liver and 4) mouse kidney tissue lysate with PPP2R1A antibody. Predicted molecular weight ~65 kDa.
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Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A431, 4) monkey COS-7, 5) rat kidney, 6) rat C6 and 7) mouse NIH 3T3 cell lysate with PPP2R1A antibody. Predicted molecular weight ~65 kDa.
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IHC staining of FFPE human liver cancer tissue with PPP2R1A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with PPP2R1A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A549 cells with PPP2R1A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human 293T cells with PPP2R1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R1A antibody.
Flow cytometry testing of mouse RAW264.7 cells with PPP2R1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R1A antibody.
Flow cytometry testing of rat C6 cells with PPP2R1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R1A antibody.
Western blot testing of 1) human HepG2, 2) rat kidney, 3) mouse liver and 4) mouse kidney tissue lysate with PPP2R1A antibody. Predicted molecular weight ~65 kDa.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A431, 4) monkey COS-7, 5) rat kidney, 6) rat C6 and 7) mouse NIH 3T3 cell lysate with PPP2R1A antibody. Predicted molecular weight ~65 kDa.
IHC staining of FFPE human liver cancer tissue with PPP2R1A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with PPP2R1A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
Optimal dilution of the PPP2R1A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform is an enzyme that in humans is encoded by the PPP2R1A gene. This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KVKEFCENLSADCRENVIMSQ from the human protein were used as the immunogen for the PPP2R1A antibody.
Limitation:
This PPP2R1A antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P30153