Spartin Antibody / SPART (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6684
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6684-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Spartin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human ThP-1 cells with Spartin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Spartin antibody.~
2 / 2
Western blot testing of 1) human HEK293, 2) human HeLa, 3) rat brain

Flow cytometry testing of human ThP-1 cells with Spartin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Spartin antibody.~
Western blot testing of 1) human HEK293, 2) human HeLa, 3) rat brain

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Spartin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Spartin is a protein that in humans is encoded by the SPART gene. This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H205-V631) was used as the immunogen for the Spartin antibody.
Limitation:
This Spartin antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q8N0X7