Tubulin-specific chaperone A Antibody / TBCA / CFA (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6687
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6687-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the TBCA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human ThP-1 cells with TBCA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TBCA antibody.~
2 / 2
Western blot testing of human 1) HEK293, 2) HeLa and 3) Raji cell lysate wi

Flow cytometry testing of human ThP-1 cells with TBCA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TBCA antibody.~
Western blot testing of human 1) HEK293, 2) HeLa and 3) Raji cell lysate wi

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TBCA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tubulin-specific chaperone A, also called Tubulin-folding cofactor A (CFA), is a protein that in humans is encoded by the TBCA gene. The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-A108) was used as the immunogen for the TBCA antibody.
Limitation:
This TBCA antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O75347