IRBIT Antibody / AHCYL1 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6710
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6710-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IRBIT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 11
IHC staining of FFPE human thyroid papillary carcinoma tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 11
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human bladder adenosquamous carcinoma tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human Hashimoto's thyroiditis tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE mouse brain tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE rat brain tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
Immunofluorescent staining of FFPE human A431 cells with IRBIT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 11
Western blot testing of 1) human HeLa, 2) human HEK293, 3) human HepG2, 4) human MCF7, 5) rat pancreas and 6) mouse pancreas tissue lysate with IRBIT antibody. Predicted molecular weight ~59 kDa.
9 / 11
Flow cytometry testing of human Caco-2 cells with IRBIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRBIT antibody.
10 / 11
Flow cytometry testing of mouse HEPA1-6 cells with IRBIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRBIT antibody.
11 / 11
Flow cytometry testing of rat RH35 cells with IRBIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRBIT antibody.
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IHC staining of FFPE human thyroid papillary carcinoma tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder adenosquamous carcinoma tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human Hashimoto's thyroiditis tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with IRBIT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with IRBIT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HEK293, 3) human HepG2, 4) human MCF7, 5) rat pancreas and 6) mouse pancreas tissue lysate with IRBIT antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of human Caco-2 cells with IRBIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRBIT antibody.
Flow cytometry testing of mouse HEPA1-6 cells with IRBIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRBIT antibody.
Flow cytometry testing of rat RH35 cells with IRBIT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRBIT antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the IRBIT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Putative adenosylhomocysteinase 2 is an enzyme that in humans is encoded by the AHCYL1 gene. The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E14-K57) was used as the immunogen for the IRBIT antibody.
Limitation:
This IRBIT antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O43865