CDH17 Antibody / Cadherin 17 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6713
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6713-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the CDH17 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human stomach cancer tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human appendicitis tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE mouse colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Immunofluorescent staining of FFPE human Caco-2 cells with CDH17 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 6
Immunofluorescent staining of FFPE human rectal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human stomach cancer tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendicitis tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with CDH17 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human rectal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDH17 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. By somatic cell hybrid analysis and fluorescence in situ hybridization, CDH17 gene is mapped to 8q22.1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E24-Q695) was used as the immunogen for the CDH17 antibody.
Limitation:
This CDH17 antibody is available for research use only.
Localization:
Cell surface, Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q12864