PLEKHA5 Antibody / PEPP-2 (Antigen affinity purified)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6723
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6723-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PLEKHA5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HeLa cells with PLEHA5 antibody (green) and phalloidin (red). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human A549, 2) human Jurkat, 3) human HeLa, 4) human HEK293, 4) rat testis and 5) mouse testis tissue lysate with PLEKHA5 antibody. Predicted molecular weight ~130 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of human JK-1 cells with PLEKHA5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLEKHA5 antibody.
❮❯

Immunofluorescent staining of FFPE human HeLa cells with PLEHA5 antibody (green) and phalloidin (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human A549, 2) human Jurkat, 3) human HeLa, 4) human HEK293, 4) rat testis and 5) mouse testis tissue lysate with PLEKHA5 antibody. Predicted molecular weight ~130 kDa (multiple isoforms).
Flow cytometry testing of human JK-1 cells with PLEKHA5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLEKHA5 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PLEKHA5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Pleckstrin homology domain-containing family A member 5, also called Phosphoinositol 3-phosphate-binding protein 2 (PEPP-2) is a protein that in humans is encoded by the PLEKHA5 gene. This gene, which encodes a transcriptional repressor, is one of two paralogous X-linked homeobox-containing genes and is highly expressed in a variety of cancers. In addition, the encoded protein associates with the cell membrane and with microtubules and is concentrated at the leading edge of migratory cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R25-Q905) was used as the immunogen for the PLEKHA5 antibody.
Limitation:
This PLEKHA5 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9HAU0