SND1 Antibody / TDRD11 (Antigen affinity purified)

Product Code: NSJ-RQ6741

Code	Size	Price

NSJ-RQ6741-100ug

100ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the TDRD11 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10

IHC staining of FFPE human tonsil tissue with TDRD11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

2 / 10

IHC staining of FFPE human rectum adenocarcinoma tissue with TDRD11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

3 / 10

IHC staining of FFPE human liver cancer tissue with TDRD11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

4 / 10

IHC staining of FFPE human metaplasia of squamous cells of the renal pelvis tissue with TDRD11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

5 / 10

IHC staining of FFPE mouse brain tissue with TDRD11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

6 / 10

7 / 10

IHC staining of FFPE rat brain tissue with TDRD11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

8 / 10

Immunofluorescent staining of FFPE human PC-3 cells with TDRD11 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

9 / 10

Western blot testing of 1) human HeLa, 2) human Raji, 3) human U-87 MG, 4) rat stomach, 5) rat pancreas, 6) mouse stomach and 7) mouse pancreas tissue with TDRD11 antibody. Predicted molecular weight ~102 kDa.

10 / 10

Flow cytometry testing of human HeLa cells with TDRD11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDRD11 antibody.

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Documents

Datasheet

Further Information

Application Details:

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

Optimal dilution of the TDRD11 antibody should be determined by the researcher.

Buffer:

Lyophilized from 1X PBS with 2% Trehalose

Description:

Staphylococcal nuclease domain-containing protein 1 also known as 100 kDa coactivator or Tudor domain-containing protein 11 (TDRD11) is a protein that in humans is encoded by the SND1 gene. This gene encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC).

Format:

Antigen affinity purified

Formulation:

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

Recombinant human protein (amino acids Q20-D204) was used as the immunogen for the TDRD11 antibody.

Limitation:

This TDRD11 antibody is available for research use only.

Localization:

Cytoplasmic, nuclear

Purity:

Affinity purified

Uniprot #:

Q7KZF4