Semaphorin 3B Antibody / SEMA3B (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6745
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6745-100ug100ug£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the SEMA3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HeLa cells with SEMA3B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human U-87 MG, 4) human K562, 5) rat kidney, 6) rat NRK, 7) mouse kidney and 8) mouse ANA-1 cell lysate with SEMA3B antibody. Predicted molecular weight ~83 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with SEMA3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEMA3B antibody.
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Immunofluorescent staining of FFPE human HeLa cells with SEMA3B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human U-87 MG, 4) human K562, 5) rat kidney, 6) rat NRK, 7) mouse kidney and 8) mouse ANA-1 cell lysate with SEMA3B antibody. Predicted molecular weight ~83 kDa.
Flow cytometry testing of human HepG2 cells with SEMA3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEMA3B antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the SEMA3B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Semaphorin-3B is a protein that in humans is encoded by the SEMA3B gene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y55-R624) was used as the immunogen for the SEMA3B antibody.
Limitation:
This SEMA3B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Uniprot #:
Q13214