NSJ Bioreagents

P Glycoprotein Antibody / MDR1 / ABCB1 (Antigen affinity purified)

Product Code:
 
NSJ-RQ6755
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ABCB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with ABCB1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HepG2 and 2) Caco-2 cell lysate with ABCB1 antibody. Expected molecular weight: 141-180 kDa depending on glycosylation level.
3 / 3
Flow cytometry testing of human 293T cells with ABCB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ABCB1 antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with ABCB1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2 and 2) Caco-2 cell lysate with ABCB1 antibody. Expected molecular weight: 141-180 kDa depending on glycosylation level.
Flow cytometry testing of human 293T cells with ABCB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ABCB1 antibody.

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CodeSizePrice
NSJ-RQ6755-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the ABCB1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
P-GP, also called ABCB1, MDR1 and PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood�brain barrier.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids TKEALDESIPPVSFWRIMK from the human protein were used as the immunogen for the ABCB1 antibody.
Limitation:
This ABCB1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P08183

Documents