GATA6 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6769
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6769-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the GATA6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A431 cells with GATA6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Immunofluorescent staining of FFPE human Caco-2 cells with GATA6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
Western blot testing of human 1) 293T, 2) MCF7 and 3) PC-3 cell lysate with GATA6 antibody. Predicted molecular weight ~60 kDa.
4 / 4
Flow cytometry testing of human U-2 OS cells with GATA6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GATA6 antibody.
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Immunofluorescent staining of FFPE human A431 cells with GATA6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human Caco-2 cells with GATA6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T, 2) MCF7 and 3) PC-3 cell lysate with GATA6 antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of human U-2 OS cells with GATA6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GATA6 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GATA6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Transcription factor GATA-6, also known as GATA-binding factor 6 (GATA6), is protein that in humans is encoded by the GATA6 gene. This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N105-L151) was used as the immunogen for the GATA6 antibody.
Limitation:
This GATA6 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q92908