NSJ Bioreagents

Giantin Antibody / GOLGB1 (Antigen affinity purified)

Product Code:
 
NSJ-RQ6771
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Giantin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 10
IHC staining of FFPE human appendiceal adenocarcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human breast cancer tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human colonic adenocarcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human esophageal squamous carcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human gastric adenocarcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human placental tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
Immunofluorescent staining of FFPE human RT4 cells with Giantin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 10
Western blot testing of human HeLa cell lysate with Giantin antibody. Predicted molecular weight ~376 kDa.
10 / 10
Flow cytometry testing of human HepG2 cells with Giantin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Giantin antibody.
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IHC staining of FFPE human appendiceal adenocarcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colonic adenocarcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric adenocarcinoma tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Giantin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human RT4 cells with Giantin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human HeLa cell lysate with Giantin antibody. Predicted molecular weight ~376 kDa.
Flow cytometry testing of human HepG2 cells with Giantin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Giantin antibody.

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CodeSizePrice
NSJ-RQ6771-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Giantin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Giantin or Golgin subfamily B member 1 is a protein that in humans is encoded by the GOLGB1 gene. GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family. GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton. It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis. GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids L153-E950) was used as the immunogen for the Giantin antibody.
Limitation:
This Giantin antibody is available for research use only.
Localization:
Cytoplasmic (Golgi)
Purity:
Antigen affinity purified
Uniprot #:
Q14789

Documents