MTHFD1L Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6778
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6778-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Monofunctional C1-tetrahydrofolate synthase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with Monofunctional C1-tetrahydrofolate synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human K562, 3) rat brain and 4) mouse brain tissue lysate with Monofunctional C1-tetrahydrofolate synthase antibody. Predicted molecular weight ~106 kDa.
3 / 3
Flow cytometry testing of human HEL cells with Monofunctional C1-tetrahydrofolate synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Monofunctional C1-tetrahydrofolate synthase antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Monofunctional C1-tetrahydrofolate synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human K562, 3) rat brain and 4) mouse brain tissue lysate with Monofunctional C1-tetrahydrofolate synthase antibody. Predicted molecular weight ~106 kDa.
Flow cytometry testing of human HEL cells with Monofunctional C1-tetrahydrofolate synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Monofunctional C1-tetrahydrofolate synthase antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Monofunctional C1-tetrahydrofolate synthase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Monofunctional C1-tetrahydrofolate synthase, mitochondrial also known as formyltetrahydrofolate synthetase, is an enzyme that in humans is encoded by the MTHFD1L gene (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like). The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R43-V833) was used as the immunogen for the Monofunctional C1-tetrahydrofolate synthase antibody.
Limitation:
This Monofunctional C1-tetrahydrofolate synthase antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q6UB35