NACC1 Antibody / NAC1 / BTBD14B (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6781
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6781-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BTBD14B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human papillary carcinoma of the breast with BTBD14B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human bladder epithelial carcinoma with BTBD14B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human renal pelvis squamous metaplasia with BTBD14B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human MCF7 cells with BTBD14B antibody (green) and phalloidin (red). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of human 1) HeLa, 2) MCF7 and 3) Caco-2 cell lysate with BTBD14B antibody. Predicted molecular weight: ~57 kDa, commonly observed at 57-70 kDa.
6 / 6
Flow cytometry testing of human K562 cells with BTBD14B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BTBD14B antibody.
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IHC staining of FFPE human papillary carcinoma of the breast with BTBD14B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder epithelial carcinoma with BTBD14B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal pelvis squamous metaplasia with BTBD14B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with BTBD14B antibody (green) and phalloidin (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) MCF7 and 3) Caco-2 cell lysate with BTBD14B antibody. Predicted molecular weight: ~57 kDa, commonly observed at 57-70 kDa.
Flow cytometry testing of human K562 cells with BTBD14B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BTBD14B antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the BTBD14B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Nucleus accumbens-associated protein 1 is a protein that in humans is encoded by the NACC1 gene. This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E114-E317) was used as the immunogen for the BTBD14B antibody.
Limitation:
This BTBD14B antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q96RE7