POLA2 Antibody / DNA polymerase alpha subunit B (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6788
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6788-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the POLA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-251 cells with POLA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= POLA2 antibody.~
2 / 2
Western blot testing of human 1) HeLa, 2) HepG2, 3) COLO-320 and 4) MCF7

Flow cytometry testing of human U-251 cells with POLA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= POLA2 antibody.~
Western blot testing of human 1) HeLa, 2) HepG2, 3) COLO-320 and 4) MCF7

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the POLA2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
DNA polymerase alpha subunit 2/B is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N267-P579) was used as the immunogen for the POLA2 antibody.
Limitation:
This POLA2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q14181