RSKB Antibody / MSK2 / RPS6KA4 (Antigen affinity purified)

Product Code: NSJ-RQ6800

Code	Size	Price

NSJ-RQ6800-100ug

100ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the RPS6KA4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10

IHC staining of FFPE human lung cancer tissue with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

2 / 10

IHC staining of FFPE moderately differentiated adenocarcinoma of the rectum with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

3 / 10

IHC staining of FFPE human bladder epithelial tissue with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

4 / 10

IHC staining of FFPE human metaplasia of squamous cells of the renal pelvis tissue with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

5 / 10

IHC staining of FFPE human placental tissue with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

6 / 10

IHC staining of FFPE mouse colon tissue with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

7 / 10

IHC staining of FFPE rat colon tissue with RPS6KA4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

8 / 10

Immunofluorescent staining of FFPE human PC-3 cells with RPS6KA4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

9 / 10

Western blot testing of human 1) HeLa, 2) 293T and 3) PC-3 cell lysate with RPS6KA4 antibody. Predicted molecular weight ~86 kDa.

10 / 10

Flow cytometry testing of human K562 cells with RPS6KA4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPS6KA4 antibody.

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Documents

Datasheet

Further Information

Application Details:

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Application Note:

Optimal dilution of the RPS6KA4 antibody should be determined by the researcher.

Buffer:

Lyophilized from 1X PBS with 2% Trehalose

Description:

Ribosomal protein S6 kinase alpha-4 is an enzyme that in humans is encoded by the RPS6KA4 gene. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.

Format:

Antigen affinity purified

Formulation:

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

Recombinant human protein (amino acids Q302-S772) was used as the immunogen for the RPS6KA4 antibody.

Limitation:

This RPS6KA4 antibody is available for research use only.

Localization:

Nuclear, cytoplasmic

Purity:

Antigen affinity purified

Uniprot #:

O75676