SREBP2 Antibody / SREBF2 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6804
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6804-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the SREBP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human MCF7 cells with SREBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human ThP-1, 2) human Jurkat, 3) human Raji, 4) human HL60, 5) rat stomach, 6) rat testis, 7) mouse stomach and 8) rat testis tissue lysate with SREBP2 antibody. Predicted molecular weight ~124 kDa but may be observed at higher molecular weights due to glycosylation. The active form of this protein can be observed at 50-68 kDa.
3 / 4
Flow cytometry testing of human K562 cells with SREBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SREBP2 antibody.
4 / 4
Flow cytometry testing of rat RH35 cells with SREBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SREBP2 antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with SREBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human ThP-1, 2) human Jurkat, 3) human Raji, 4) human HL60, 5) rat stomach, 6) rat testis, 7) mouse stomach and 8) rat testis tissue lysate with SREBP2 antibody. Predicted molecular weight ~124 kDa but may be observed at higher molecular weights due to glycosylation. The active form of this protein can be observed at 50-68 kDa.
Flow cytometry testing of human K562 cells with SREBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SREBP2 antibody.
Flow cytometry testing of rat RH35 cells with SREBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SREBP2 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SREBP2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Sterol regulatory element-binding protein 2 (SREBP-2) also known as sterol regulatory element binding transcription factor 2 (SREBF2) is a protein that in humans is encoded by the SREBF2 gene. This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R371-L409) was used as the immunogen for the SREBP2 antibody.
Limitation:
This SREBP2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q12772