Sorcin Antibody / SRI (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6805
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6805-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Sorcin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human SiHa cells with Sorcin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) rat brain and 3) mouse brain tissue lysate with Sorcin antibody. Predicted molecular weight ~22 kDa, routinely observed at 22-29 kDa.
3 / 3
Flow cytometry testing of human 293T cells with Sorcin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sorcin antibody.
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Immunofluorescent staining of FFPE human SiHa cells with Sorcin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) rat brain and 3) mouse brain tissue lysate with Sorcin antibody. Predicted molecular weight ~22 kDa, routinely observed at 22-29 kDa.
Flow cytometry testing of human 293T cells with Sorcin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sorcin antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Sorcin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Sorcin is a protein that in humans is encoded by the SRI gene. It is mapped to 7q21.1. This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D121-A161) was used as the immunogen for the Sorcin antibody.
Limitation:
This Sorcin antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P30626