TRAP-1 Antibody / HSP75 (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6811
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6811-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP75 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human HL60 cells with HSP75 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP75 antibody.~
2 / 2
Western blot testing of 1) human K562, 2) human HL60, 3) human 293T, 4) hu

Flow cytometry testing of human HL60 cells with HSP75 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP75 antibody.~
Western blot testing of 1) human K562, 2) human HL60, 3) human 293T, 4) hu

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HSP75 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Heat shock protein 75 kDa, mitochondrial is a protein that in humans is encoded by the TRAP1 gene. It is mapped to 16p13.3. This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. And this protein may function in regulating cellular stress responses. In addition, it was found that TRAP1 interacted with the N-terminal half of TNFR1. Also, TRAP1 interacted with the C-terminal ends of the proteins encoded by both multiple exostoses-causing genes, EXT1 and EXT2, but not with EXTL1 or EXTL3.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A618-H704) was used as the immunogen for the HSP75 antibody.
Limitation:
This HSP75 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q12931