WNT8B Antibody (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6818
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6818-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the WNT8B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with WNT8B antibody. HIER: steam section in pH6 citrate buffer for 20 min.~
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Western blot testing of human RT4 cell lysate with WNT8B antibody. Predicted molecular weight ~39 kDa.

Immunofluorescent staining of FFPE human MCF7 cells with WNT8B antibody. HIER: steam section in pH6 citrate buffer for 20 min.~
Western blot testing of human RT4 cell lysate with WNT8B antibody. Predicted molecular weight ~39 kDa.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the WNT8B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein Wnt-8b is a protein that in humans is encoded by the WNT8B gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A206-R340) was used as the immunogen for the WNT8B antibody.
Limitation:
This WNT8B antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q93098